Human Prolactin Receptor - PRLR ELISA Kit

Mfr.No.

E4610Hu

Sensitivity

0.022ng/ml

Detection Range

0.05-20ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

PRLR;PRL-R;Prolactin receptor

Molecular Weight

69506

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E4610Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1.8	0.09	4.8
Intra-Assay	2	18	3.2	0.13	4
Intra-Assay	3	18	1	0.06	5.9

Background

This is a receptor for the anterior pituitary hormone prolactin (PRL). Acts as a prosurvival factor for spermatozoa by inhibiting sperm capacitation through suppression of SRC kinase activation and stimulation of AKT. Isoform 4 is unable to transduce prolactin signaling. Isoform 6 is unable to transduce prolactin signaling.

Protocol

These standard curves of E4610Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.204	2.134	2.103
	2.063
6ng/ml	1.701	1.796	1.766
	1.892
3ng/ml	1.177	1.171	1.141
	1.165
1.5ng/ml	0.708	0.668	0.638
	0.629
0.75ng/ml	0.4	0.402	0.371
	0.403
0ng/ml	0.034	0.031	0
	0.027

Gene ID	5618
UniProt ID	P16471

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105916	E4610Hu	48T	$320		Add to Cart
BHE12105916	E4610Hu	96T	$458		Add to Cart

Human Prolactin Receptor - PRLR ELISA Kit

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