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Home / ELISA Kit

Human Prohibitin (PHB) ELISA Kit

SKU : BHE13703438

Prohibitin is a protein which in humans is encoded by the PHB gene. The Phb gene has also been described in animals, fungi, plants and unicellular eukaryotes. Prohibitins are divided in two classes, termed Type-I and Type-II prohibitins, based on their similarity to yeast PHB1 or PHB2, respectively.
Each organism has at least one copy of each type of prohibitin gene.Prohibitins are evolutionarily conserved genes that are ubiquitously expressed. The human prohibitin gene, located on the BRCA1 chromosome region 17q21, was originally thought to be a negative regulator of cell proliferation and a tumor suppressor. This anti-proliferative activity was later attributed to the 3' UTR of the PHB gene, and not to the actual protein.

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Mfr.No.	KTE61222
Description	This Human Prohibitin (PHB) ELISA Kit employs a two-site sandwich ELISA to quantitate PHB.
Alternative Names	PHB; PHB1;
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Prohibitin microplate Human Prohibitin standard Human Prohibitin detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Prohibitin (PHB) ELISA Kit has high sensitivity and excellent specificity for detection of Human PHB. No significant cross-reactivity or interference between Human PHB and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Prohibitin is a protein which in humans is encoded by the PHB gene. The Phb gene has also been described in animals, fungi, plants and unicellular eukaryotes. Prohibitins are divided in two classes, termed Type-I and Type-II prohibitins, based on their similarity to yeast PHB1 or PHB2, respectively. Each organism has at least one copy of each type of prohibitin gene.Prohibitins are evolutionarily conserved genes that are ubiquitously expressed. The human prohibitin gene, located on the BRCA1 chromosome region 17q21, was originally thought to be a negative regulator of cell proliferation and a tumor suppressor. This anti-proliferative activity was later attributed to the 3' UTR of the PHB gene, and not to the actual protein.
Notes	This Human Prohibitin (PHB) ELISA Kit employs a two-site sandwich ELISA to quantitate PHB in samples. An antibody specific for PHB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPHB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PHB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PHB bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	5245
Antigen	PHB
Tag	PHB
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Prohibitin (PHB) ELISA Kit employs a two-site sandwich ELISA to quantitate PHB in samples. An antibody specific for PHB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPHB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PHB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PHB bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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