Human Programmed Death Ligand-1(PD-L1/CD274) ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E13644h

Sensitivity

3.9 pg/mL

Detection Range

15.6 pg/mL-1000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

CD274 molecule, B7-H, B7H1, MGC142294, MGC142296, PD-L1, PDCD1L1, PDCD1LG1, PDL1, CD274 antigen, programmed cell death 1 ligand 1

Recovery

The recovery of human PAWR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	90-102
EDTA plasma (n=4)	94	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human PAWR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-91
1:2	Average %	90
	Range %	84-98
1:4	Average %	92
	Range %	86-100
1:8	Average %	95
	Range %	88-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q9NZQ7

Applications	ELISA
Research Area	CD274

Storage	Store at 2-8°C. Please refer to protocol.

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