Human Programmed Cell Death Protein 4

Mfr.No.

E4077hu

Sensitivity

0.025ng/ml

Detection Range

0.05-20ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Human Programmed Cell Death Protein 4 (also known as PDCD4) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Neoplastic transformation inhibitor protein;Nuclear antigen H731-like;PDCD 4;PDCD4;Programmed cell death protein 4;Protein 197/15a

Molecular Weight

51735

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E4077hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1	0.06	6.1
Intra-Assay	2	18	6.3	0.37	5.8
Intra-Assay	3	18	2.7	0.18	6.5

Background

Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA (By similarity).

Protocol

These standard curves of E4077hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.273	2.258	2.214
	2.244
6ng/ml	1.756	1.745	1.701
	1.734
3ng/ml	1.115	1.141	1.097
	1.166
1.5ng/ml	0.635	0.689	0.645
	0.743
0.75ng/ml	0.46	0.464	0.42
	0.468
0ng/ml	0.026	0.044	0
	0.061

Gene ID	27250
UniProt ID	Q53EL6

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105490	E4077hu	48T	$320		Add to Cart
BHE12105490	E4077hu	96T	$458		Add to Cart

Human Programmed Cell Death Protein 4 - PDCD4 ELISA Kit

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