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Home / ELISA Kit

Human Programmed cell death 6-interacting protein (PDCD6IP) ELISA Kit

SKU : BHE13703491

Programmed cell death 6-interacting protein is a protein thought to participate in programmed cell death. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner.
This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization which may be partly responsible for the protection against cell death.Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.

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Mfr.No.	KTE61275
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Programmed cell death 6-interacting protein (PDCD6IP) ELISA Kit employs a two-site sandwich ELISA to quantitate PDCD6IP.
Alternative Names	PDCD6IP; AIP1; Alix; DRIP4; HP95; MGC17003; ALG-2 interacting protein 1; ALG-2 interacting protein X; apoptosis-linked gene 2-interacting protein X; dopamine receptor interacting protein 4; programmed cell death
Detection Method	Colorimetric
Kit Components	Human Programmed cell death 6-interacting protein microplate Human Programmed cell death 6-interacting protein standard Human Programmed cell death 6-interacting protein detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Programmed cell death 6-interacting protein (PDCD6IP) ELISA Kit has high sensitivity and excellent specificity for detection of Human PDCD6IP. No significant cross-reactivity or interference between Human PDCD6IP and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Programmed cell death 6-interacting protein is a protein thought to participate in programmed cell death. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization which may be partly responsible for the protection against cell death.Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Notes	This Human Programmed cell death 6-interacting protein (PDCD6IP) ELISA Kit employs a two-site sandwich ELISA to quantitate PDCD6IP in samples. An antibody specific for PDCD6IP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDCD6IP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDCD6IP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDCD6IP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	10015

Applications	ELISA
Applications Notes	This Human Programmed cell death 6-interacting protein (PDCD6IP) ELISA Kit employs a two-site sandwich ELISA to quantitate PDCD6IP in samples. An antibody specific for PDCD6IP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDCD6IP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDCD6IP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDCD6IP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703491	KTE61275	48 T	$339		Add to Cart
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