Human Programmed cell death 6-interacting protein(PDCD6IP) ELISA kit

Human Programmed cell death 6-interacting protein(PDCD6IP) ELISA kit

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Mfr.No.

CSB-EL017673HU

Alternative Names

programmed cell death 6 interacting protein, AIP1, Alix, DRIP4, HP95, MGC17003, ALG-2 interacting protein 1, ALG-2 interacting protein X, apoptosis-linked gene 2-interacting protein X, dopamine receptor interacting protein 4, programmed cell death

Reactivity

Human

Detection Range

47 pg/mL-3000 pg/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human IL-28B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	100	95-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IL-28B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-92
1:2	Average %	96
	Range %	90-100
1:4	Average %	100
	Range %	95-105
1:8	Average %	96
	Range %	93-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
100	2.224	2.239	2.232	2.047
50	1.509	1.534	1.522	1.337
25	1.023	1.036	1.030	0.845
12.5	0.688	0.699	0.694	0.509
6.25	0.474	0.490	0.482	0.297
3.12	0.387	0.374	0.381	0.196
1.56	0.281	0.297	0.289	0.104
0	0.184	0.186	0.185	?

Species

Homo sapiens

Sensitivity

11.7 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q8WUM4
Antigen	programmed cell death 6 interacting protein

Applications

ELISA

Research Area

PDCD6IP

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503894	CSB-EL017673HU	96T	$695	Add to Cart
BHE10503894	CSB-EL017673HU	5x96T	$3336	Add to Cart
BHE10503894	CSB-EL017673HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.