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Home / ELISA Kit

Human Progestagen Associated Endometrial Protein (PAEP) ELISA Kit

SKU : BHE13703534

Glycodelin is a member of the kernel lipocalin superfamily whose members share relatively low sequence similarity but have highly conserved exon/intron structure and three-dimensional protein folding. Most lipocalins are clustered on the long arm of chromosome 9.
The encoded glycoprotein has been previously referred to as pregnancy-associated endometrial alpha-2-globulin, placental protein 14, and glycodelin, but has been officially named progestagen-associated endometrial protein. Three distinct forms, with identical protein backbones but different glycosylation profiles, are found in amniotic fluid, follicular fluid and seminal plasma of the reproductive system.

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Mfr.No.	KTE61318
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Progestagen Associated Endometrial Protein (PAEP) ELISA Kit employs a two-site sandwich ELISA to quantitate PAEP.
Alternative Names	PAEP; RP11-98L5.3; GD; GdA; GdF; GdS; MGC138509; MGC142288; PAEG; PEP; PP14; OTTHUMP00000022549; PP14 protein (placental protein 14); alpha uterine protein; glycodelin; glycodelin-A; glycodelin-F; glycodelin-S; ; Glycodelin; Placental Protein 14; Pregnancy-Associated Endometrial Alpha-2-Globulin; Alpha Uterine Protein
Detection Method	Colorimetric
Kit Components	Human Progestagen Associated Endometrial Protein microplate Human Progestagen Associated Endometrial Protein standard Human Progestagen Associated Endometrial Protein detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Progestagen Associated Endometrial Protein (PAEP) ELISA Kit has high sensitivity and excellent specificity for detection of Human PAEP. No significant cross-reactivity or interference between Human PAEP and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Glycodelin is a member of the kernel lipocalin superfamily whose members share relatively low sequence similarity but have highly conserved exon/intron structure and three-dimensional protein folding. Most lipocalins are clustered on the long arm of chromosome 9. The encoded glycoprotein has been previously referred to as pregnancy-associated endometrial alpha-2-globulin, placental protein 14, and glycodelin, but has been officially named progestagen-associated endometrial protein. Three distinct forms, with identical protein backbones but different glycosylation profiles, are found in amniotic fluid, follicular fluid and seminal plasma of the reproductive system.
Notes	This Human Progestagen Associated Endometrial Protein (PAEP) ELISA Kit employs a two-site sandwich ELISA to quantitate PAEP in samples. An antibody specific for PAEP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPAEP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PAEP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PAEP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Progestagen Associated Endometrial Protein (PAEP) ELISA Kit employs a two-site sandwich ELISA to quantitate PAEP in samples. An antibody specific for PAEP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPAEP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PAEP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PAEP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703534	KTE61318	48 T	$359		Add to Cart
BHE13703534	KTE61318	96 T	$619		Add to Cart
BHE13703534	KTE61318	96 Tx5	$2809		Add to Cart
BHE13703534	KTE61318	96 Tx50	$26209		Add to Cart

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