Human Procollagen-lysine - 2-oxoglutarate 5-dioxygenase 2

Mfr.No.

E5919Hu

Alternative Names

LH2;Lysyl hydroxylase 2;PLOD 2;PLOD2;Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2

Reactivity

Human

Detection Range

5-1000ng/L

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

84686

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

2.24ng/L

Background

Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links.

Gene ID	5352
UniProt ID	O00469

Research Area

Signal Transduction

Protocol

These standard curves of E5919Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
640ng/L	1.951	2.123	2.079
	2.294
320ng/L	1.343	1.344	1.301
	1.346
160ng/L	0.598	0.634	0.591
	0.67
80ng/L	0.318	0.36	0.317
	0.402
40ng/L	0.18	0.168	0.125
	0.157
0ng/L	0.04	0.043	0
	0.046

Precision

We measured random samples of E5919Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	414.9	15.47	3.7
Intra-Assay	2	18	94.1	4.96	5.3
Intra-Assay	3	18	213.2	10.95	5.1

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107281	E5919Hu	48T	$320		Add to Cart
BHE12107281	E5919Hu	96T	$458		Add to Cart

Human Procollagen-lysine - 2-oxoglutarate 5-dioxygenase 2 - PLOD2 ELISA Kit

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