Human procollagen I N-terminal peptide,PINP ELISA Kit

Human procollagen I N-terminal peptide,PINP ELISA Kit

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Mfr.No.

CSB-E11226h

Alternative Names

procollagen I N-terminal peptide,PINP, procollagen 1 N-terminal peptide,P1NP,_x000D_ procollagen I N-terminal peptide,PINP, procollagen I N-terminal peptide,PINP, procollagen 1 N-terminal peptide,P1NP,_x000D_ procollagen I N-terminal peptide,PINP

Reactivity

Human

Detection Range

18.75 pg/mL-1200 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rat IL-22 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	95-105
EDTA plasma (n=4)	104	92-109

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-22 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	83-94
1:2	Average %	99
	Range %	90-102
1:4	Average %	93
	Range %	85-97
1:8	Average %	92
	Range %	84-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.120	2.147	2.134	1.958
500	1.493	1.486	1.490	1.314
250	1.029	1.105	1.067	0.891
125	0.658	0.673	0.666	0.490
62.5	0.456	0.444	0.450	0.274
31.2	0.346	0.358	0.352	0.176
15.6	0.270	0.262	0.266	0.090
0	0.174	0.178	0.176

Species

Homo sapiens

Sensitivity

4.68 pg/mL

Limit Of Detection

450 nm

Antigen	procollagen Ⅰ N-terminal peptide,PⅠNP

Applications

ELISA

Research Area

PINP

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Effect of chronic activity-based therapy on bone mineral density and bone turnover in persons with spinal cord injury Astorino TA et al,Eur J Appl Physiol,2013 Dec;113(12):3027-37. Physical activity and hypocaloric diet recovers osteoblasts homeostasis in women affected by abdominal obesity Viviana M.et al,Springer US,Dec 16 Spine bone mineral density increases after 6 months of exclusive lactation, even in women who keep breastfeeding Cooke-Hubley S.et al,Arch Osteoporos,Aug 16

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SKU	Supplier No.	Size	Your price
BHE10503872	CSB-E11226h	96T	$695	Add to Cart
BHE10503872	CSB-E11226h	5x96T	$3336	Add to Cart
BHE10503872	CSB-E11226h	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.