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Home / ELISA Kit

Human Procollagen C-endopeptidase enhancer 2 (PCOLCE2) ELISA Kit

SKU : BHE13703505

PCOLCE2 lies 5.2 kb and 102.5 kb centromeric to the transient receptor potential channel-1 (TRPC1) and plastin-1 (PLS1), respectively. The deduced 415-amino acid protein contains an N-terminal signal sequence, 2 CUB domains, and an NTR domain. It also contains a putative myristoylation site, several potential phosphorylation sites, a putative glycosylation site, and an RGD site. PCOLCE2 shares about 43% sequence identity with the PCOLCE protein .
The secreted PCOLCE2 protein is calculated to have a molecular mass of about 47 kD. Northern blot analysis detected ubiquitous expression of a 2-kb transcript, with highest expression in heart, placenta, and trabecular meshwork, and very low expression in brain. Western blot analysis revealed a 52-kD form of PCOLCE2 in human fibroblast cells.

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Mfr.No.	KTE61289
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Procollagen C-endopeptidase enhancer 2 (PCOLCE2) ELISA Kit employs a two-site sandwich ELISA to quantitate PCOLCE2.
Alternative Names	PCOLCE2; PCPE2;
Detection Method	Colorimetric
Kit Components	Human Procollagen C-endopeptidase enhancer 2 microplate Human Procollagen C-endopeptidase enhancer 2 standard Human Procollagen C-endopeptidase enhancer 2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Procollagen C-endopeptidase enhancer 2 (PCOLCE2) ELISA Kit has high sensitivity and excellent specificity for detection of Human PCOLCE2. No significant cross-reactivity or interference between Human PCOLCE2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	PCOLCE2 lies 5.2 kb and 102.5 kb centromeric to the transient receptor potential channel-1 (TRPC1) and plastin-1 (PLS1), respectively. The deduced 415-amino acid protein contains an N-terminal signal sequence, 2 CUB domains, and an NTR domain. It also contains a putative myristoylation site, several potential phosphorylation sites, a putative glycosylation site, and an RGD site. PCOLCE2 shares about 43% sequence identity with the PCOLCE protein . The secreted PCOLCE2 protein is calculated to have a molecular mass of about 47 kD. Northern blot analysis detected ubiquitous expression of a 2-kb transcript, with highest expression in heart, placenta, and trabecular meshwork, and very low expression in brain. Western blot analysis revealed a 52-kD form of PCOLCE2 in human fibroblast cells.
Notes	This Human Procollagen C-endopeptidase enhancer 2 (PCOLCE2) ELISA Kit employs a two-site sandwich ELISA to quantitate PCOLCE2 in samples. An antibody specific for PCOLCE2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPCOLCE2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCOLCE2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCOLCE2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	26577

Applications	ELISA
Applications Notes	This Human Procollagen C-endopeptidase enhancer 2 (PCOLCE2) ELISA Kit employs a two-site sandwich ELISA to quantitate PCOLCE2 in samples. An antibody specific for PCOLCE2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPCOLCE2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCOLCE2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCOLCE2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703505	KTE61289	48 T	$339		Add to Cart
BHE13703505	KTE61289	96 T	$589		Add to Cart
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BHE13703505	KTE61289	96 Tx50	$24569		Add to Cart

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