Human PROC/Protein C ELISA Kit PicoKine®

Mfr.No.

EK1975

Sensitivity

<0.1 ng/ml

Detection Range

0.78 ng/ml - 50 ng/ml

Reactivity

Human

Sample Volume

Concentration (ng/ml)	0	0.78	1.56	3.12	6.25	12.5	25	50
O.D.	0.017	0.135	0.233	0.445	0.857	1.343	1.813	2.017

Description

Human PROC/Protein C ELISA Kit PicoKine® (96 Tests). Quantitate Human Proc in cell culture supeatants, serum and plasma (heparin, EDTA) and urine. Sensitivity: 0.1ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Vitamin K-dependent protein C; Anticoagulant protein C; Autoprothrombin IIA; Blood coagulation factor XIV; Proc

Immunogen

Expression system for standard: NS0; Immunogen sequence: A43-P461

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human PROC/Protein C

Subcellular Localization

Secreted. Endoplasmic reticulum. Golgi apparatus.

Sample Type

Cell culture supeatants, Serum, Plasma, Urine

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	1223	5529	25231	1347	6539	25258
Standard deviation	52.59	359.39	1185.86	83.51	320.41	1793.32
CV (%)	4.3%	6.5%	4.7%	6.2%	4.9%	7.1%

Protein Function

Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids. Exerts a protective effect on the endothelial cell barrier function.

Background

Protein C (PROC), also called PC, is a zymogenic (inactive) protein, the activated form of which plays an important role in regulating blood clotting, inflammation, cell death and maintaining the permeability of blood vessel walls in humans and other animals. The PROC gene is mapped to 2q14.3. This gene encodes a vitamin K-dependent plasma glycoprotein. The encoded protein is cleaved to its activated form by the thrombinthrombomodulin complex. This activated form contains a serine protease domain and functions in degradation of the activated forms of coagulation factors V and VIII. Mutations in this gene have been associated with thrombophilia due to protein C deficiency, neonatal purpura fulminans, and recurrent venous thrombosis.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	1223	1177	1347	1353	1275	76.77	6.0%
Sample 2	5529	5748	6539	6544	6090	458.09	7.5%
Sample 3	25231	23258	22794	25529	24203	1193.03	4.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	5624
Gene Name	Proc
UniProt ID	P33587
Tissue Specificity	Plasma; synthesized in the liver.

Applications	ELISA
Research Area	Cytoskeleton/ECM,ECM Proteins,Extracellular Matrix,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001476	EK1975	96 wells/kit	$499		Add to Cart

Human PROC/Protein C ELISA Kit PicoKine®

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