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Home / ELISA Kit

Human Probable E3 ubiquitin-protein ligase makorin-3 (MKRN3) ELISA Kit

SKU : BHE13703821

Probable E3 ubiquitin-protein ligase makorin-3 encoded by MKRN3 contains a RING (C3HC4) zinc finger motif and several C3H zinc finger motifs. MKRN3 is intronless and imprinted, with expression only from the paternal allele. Disruption of the imprinting at this locus may contribute to Prader-Willi syndrome. An antisense RNA of unknown function has been found overlapping MKRN3.

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Mfr.No.	KTE61606
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Probable E3 ubiquitin-protein ligase makorin-3 (MKRN3) ELISA Kit employs a two-site sandwich ELISA to quantitate MKRN3.
Alternative Names	MKRN3; D15S9; MGC88288; RNF63; ZFP127; ZNF127; zinc finger protein 127
Detection Method	Colorimetric
Kit Components	Human Probable E3 ubiquitin-protein ligase makorin-3 microplate Human Probable E3 ubiquitin-protein ligase makorin-3 standard Human Probable E3 ubiquitin-protein ligase makorin-3 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Probable E3 ubiquitin-protein ligase makorin-3 (MKRN3) ELISA Kit has high sensitivity and excellent specificity for detection of Human MKRN3. No significant cross-reactivity or interference between Human MKRN3 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Probable E3 ubiquitin-protein ligase makorin-3 encoded by MKRN3 contains a RING (C3HC4) zinc finger motif and several C3H zinc finger motifs. MKRN3 is intronless and imprinted, with expression only from the paternal allele. Disruption of the imprinting at this locus may contribute to Prader-Willi syndrome. An antisense RNA of unknown function has been found overlapping MKRN3.
Notes	This Human Probable E3 ubiquitin-protein ligase makorin-3 (MKRN3) ELISA Kit employs a two-site sandwich ELISA to quantitate MKRN3 in samples. An antibody specific for MKRN3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMKRN3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MKRN3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MKRN3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	7681

Applications	ELISA
Applications Notes	This Human Probable E3 ubiquitin-protein ligase makorin-3 (MKRN3) ELISA Kit employs a two-site sandwich ELISA to quantitate MKRN3 in samples. An antibody specific for MKRN3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMKRN3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MKRN3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MKRN3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703821	KTE61606	48 T	$339		Add to Cart
BHE13703821	KTE61606	96 T	$589		Add to Cart
BHE13703821	KTE61606	96 Tx5	$2629		Add to Cart
BHE13703821	KTE61606	96 Tx50	$24569		Add to Cart

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