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Home / ELISA Kit

Human Pro-melanin-concentrating hormone (PMCH) ELISA Kit

SKU : BHE13703416

The melanin-concentrating hormone (MCH) is a cyclic neuropeptide isolated initially from salmon pituitary gland and later from rat hypothalamus. In mammals, MCH perikarya are confined largely to the lateral hypothalamus and zona incerta area with extensive neuronal projections throughout the brain, including the neurohypophysis. The anatomic distribution suggests a neurotransmitter or neuromodulator role for MCH in a broad array of neuronal functions directed toward the regulation of goal-directed behavior, such as food intake, and general arousal. MCH and 2 other putative neuropeptides, NEI and NGE, are encoded by the same precursor and appear colocalized in nerve cells and in many instances within the projections. The precursor is designated pro-melanin-concentrating hormone (PMCH).

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Mfr.No.	KTE61200
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Pro-melanin-concentrating hormone (PMCH) ELISA Kit employs a two-site sandwich ELISA to quantitate PMCH.
Alternative Names	PMCH; MCH;
Detection Method	Colorimetric
Kit Components	Human Pro-melanin-concentrating hormone microplate Human Pro-melanin-concentrating hormone standard Human Pro-melanin-concentrating hormone detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Pro-melanin-concentrating hormone (PMCH) ELISA Kit has high sensitivity and excellent specificity for detection of Human PMCH. No significant cross-reactivity or interference between Human PMCH and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The melanin-concentrating hormone (MCH) is a cyclic neuropeptide isolated initially from salmon pituitary gland and later from rat hypothalamus. In mammals, MCH perikarya are confined largely to the lateral hypothalamus and zona incerta area with extensive neuronal projections throughout the brain, including the neurohypophysis. The anatomic distribution suggests a neurotransmitter or neuromodulator role for MCH in a broad array of neuronal functions directed toward the regulation of goal-directed behavior, such as food intake, and general arousal. MCH and 2 other putative neuropeptides, NEI and NGE, are encoded by the same precursor and appear colocalized in nerve cells and in many instances within the projections. The precursor is designated pro-melanin-concentrating hormone (PMCH).
Notes	This Human Pro-melanin-concentrating hormone (PMCH) ELISA Kit employs a two-site sandwich ELISA to quantitate PMCH in samples. An antibody specific for PMCH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPMCH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PMCH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PMCH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	449022

Applications	ELISA
Applications Notes	This Human Pro-melanin-concentrating hormone (PMCH) ELISA Kit employs a two-site sandwich ELISA to quantitate PMCH in samples. An antibody specific for PMCH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPMCH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PMCH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PMCH bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703416	KTE61200	48 T	$339		Add to Cart
BHE13703416	KTE61200	96 T	$589		Add to Cart
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BHE13703416	KTE61200	96 Tx50	$24569		Add to Cart

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