Human Pro-collagen I alpha 1/COL1A1 ELISA Kit PicoKine®

Mfr.No.

EK1692

Sensitivity

<10 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.026	0.09	0.119	0.215	0.374	0.716	1.249	2.052

Description

Human Pro-collagen I?1/COL1A1 ELISA Kit PicoKine® (96 Tests). Quantitate Human COL1A1 in cell culture supeatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Collagen alpha-1 (I) chain; Alpha-1 type I collagen; COL1A1

Immunogen

Expression system for standard: CHO; Immunogen sequence: Q23-K277 + G1094-L1464

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human COL1A1

Subcellular Localization

Secreted, extracellular space, extracellular matrix

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin, EDTA or citrate

Activating Reagent

Aliquot 0.1 ml per well of the 4,000 pg/ml, 2,000 pg/ml, 1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml human COL1A1 standard solutions into the precoated 96-well plate. Add 0.1 ml of the sample diluent buffer into the control well (Zero well). Add 0.1 ml of each properly diluted sample of human cell culture supeatants, serum or plasma (heparin, EDTA, citrate) to each empty well. See Sample Dilution Guideline above for details. It is recommended that each human COL1A1 standard solution and each sample be measured in duplicate.

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	105	360	1669	78	251	1684
Standard deviation	3.03	19.01	123.34	5.65	11.55	105.59
CV(%)	2.9%	5.4%	7.4%	7.2%	4.6%	6.3%

Protein Function

Type I collagen is a member of group I collagen (fibrillar forming collagen).

Background

Collagen, type I, alpha 1, also known as COL1A1, is a human gene that encodes the major component of type I collagen, the fibrillar collagen found in most connective tissues, including cartilage. This gene is mapped to 17q21.33. And this gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, coea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	95	94	96	98	95	1.47	1.5%
Sample 2	317	320	299	298	308	10.06	3.2%
Sample 3	1043	1012	1051	1042	1037	14.84	1.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	P02452
Gene Name	COL1A1
UniProt ID	P02452
Tissue Specificity	Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.

Applications	ELISA
Research Area	Collagen,Cytoskeleton/ECM,ECM Proteins,Extracellular Matrix,Mesenchymal Stem Cells,Signal Transduction,Stem Cells

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001357	EK1692	96 wells/kit	$450		Add to Cart

Human Pro-collagen I alpha 1/COL1A1 ELISA Kit PicoKine®

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