Matrices listed below were spiked with certain level of Pro-ADM and the recovery rates were calculated by comparing the measured value to the expected amount of Pro-ADM in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-105
97
EDTA plasma(n=5)
86-99
92
heparin plasma(n=5)
94-101
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pro-ADM and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Pro-ADM ELISA Kit allows for the in vitro quantitative determination of Pro-ADM concentrations in serum, plasma, tissue homogenates and other biological fluids.