Matrices listed below were spiked with certain level of PRKG1 and the recovery rates were calculated by comparing the measured value to the expected amount of PRKG1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
92-102
98
EDTA plasma(n=5)
85-104
95
heparin plasma(n=5)
88-104
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PRKG1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
PRKG1 ELISA Kit allows for the in vitro quantitative determination of PRKG1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human PRKG1(Protein Kinase, cGMP Dependent Type I) ELISA Kit