Matrices listed below were spiked with certain level of PRKAR2A and the recovery rates were calculated by comparing the measured value to the expected amount of PRKAR2A in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
1-104
48
EDTA plasma(n=5)
4-102
37
heparin plasma(n=5)
12-61
34
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PRKAR2A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
10-74%
21-69%
14-81%
EDTA plasma(n=5)
11-92%
34-89%
33-99%
heparin plasma(n=5)
23-97%
28-86%
20-58%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
UniProt ID
P13861
Applications
ELISA
Applications Notes
PRKAR2A ELISA Kit allows for the in vitro quantitative determination of PRKAR2A concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human PRKAR2A (cAMP-dependent protein kinase type II-alpha regulatory subunit) ELISA Kit