Matrices listed below were spiked with certain level of PRKACA and the recovery rates were calculated by comparing the measured value to the expected amount of PRKACA in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-103
95
EDTA plasma(n=5)
87-100
93
heparin plasma(n=5)
85-98
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PRKACA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
PRKACA/cAMP-dependent protein kinase catalytic subunit alpha/PKA C-alpha
Notes
For Research Use Only
UniProt ID
P17612
Antigen
PRKACA
Applications
ELISA
Applications Notes
PRKACA ELISA Kit allows for the in vitro quantitative determination of PRKACA concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human PRKACA(cAMP-dependent protein kinase catalytic subunit alpha) ELISA Kit