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Home / ELISA Kit

Human Prickle-like protein 3 (PRICKLE3) ELISA Kit

SKU : BHE13703376

In the study of a gene-rich region of the X chromosome, Xp11.23-p11.22, Fisher et al. (1997) identified a novel locus consisting of 8 exons in a 12.5-kb interval between the A4 and the SYP loci. This gene, called LMO6, was predicted to encode a product of 407 amino acids with significant homology to proteins containing LIM domains, in particular, mouse testin. The LIM domain is a cysteine-rich sequence motif that binds zinc atoms to form a specific protein-binding interface in protein-protein interactions. Like testin, the LMO6 product contains 3 such domains. Using an RT-PCR approach, they were able to amplify a 450-bp product spanning exons 5 to 7 of the LMO6 transcript from lymphoblastoid RNA. This confirmed that the LMO6 locus is indeed transcribed and unlikely to be a pseudogene.

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Mfr.No.	KTE61160
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Prickle-like protein 3 (PRICKLE3) ELISA Kit employs a two-site sandwich ELISA to quantitate PRICKLE3.
Alternative Names	PRICKLE3; LMO6; LIM domain only 6
Detection Method	Colorimetric
Kit Components	Human Prickle-like protein 3 microplate Human Prickle-like protein 3 standard Human Prickle-like protein 3 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Prickle-like protein 3 (PRICKLE3) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRICKLE3. No significant cross-reactivity or interference between Human PRICKLE3 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	In the study of a gene-rich region of the X chromosome, Xp11.23-p11.22, Fisher et al. (1997) identified a novel locus consisting of 8 exons in a 12.5-kb interval between the A4 and the SYP loci. This gene, called LMO6, was predicted to encode a product of 407 amino acids with significant homology to proteins containing LIM domains, in particular, mouse testin. The LIM domain is a cysteine-rich sequence motif that binds zinc atoms to form a specific protein-binding interface in protein-protein interactions. Like testin, the LMO6 product contains 3 such domains. Using an RT-PCR approach, they were able to amplify a 450-bp product spanning exons 5 to 7 of the LMO6 transcript from lymphoblastoid RNA. This confirmed that the LMO6 locus is indeed transcribed and unlikely to be a pseudogene.
Notes	This Human Prickle-like protein 3 (PRICKLE3) ELISA Kit employs a two-site sandwich ELISA to quantitate PRICKLE3 in samples. An antibody specific for PRICKLE3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRICKLE3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRICKLE3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRICKLE3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	4007

Applications	ELISA
Applications Notes	This Human Prickle-like protein 3 (PRICKLE3) ELISA Kit employs a two-site sandwich ELISA to quantitate PRICKLE3 in samples. An antibody specific for PRICKLE3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRICKLE3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRICKLE3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRICKLE3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703376	KTE61160	48 T	$339		Add to Cart
BHE13703376	KTE61160	96 T	$589		Add to Cart
BHE13703376	KTE61160	96 Tx5	$2629		Add to Cart
BHE13703376	KTE61160	96 Tx50	$24569		Add to Cart

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