Human PRELP/Prolargin ELISA Kit PicoKine®

Mfr.No.

EK2110

Sensitivity

<50 pg/ml

Detection Range

93.8 pg/ml - 6,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	93.8	187.5	375	750	1500	3000	6000
O.D.	0.055	0.159	0.274	0.358	0.707	0.906	1.686	2.029

Description

Human PRELP ELISA Kit PicoKine® (96 Tests). Quantitate Human PRELP in cell culture supeatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Prolargin; Proline-arginine-rich end leucine-rich repeat protein; PRELP; SLRR2A

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q21-I382

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human PRELP

Subcellular Localization

extracellular matrix

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	102	740	3579	195	936	3857
Standard deviation	5.30	48.10	204.00	13.46	54.29	242.99
CV (%)	5.2%	6.5%	5.7%	6.9%	5.8%	6.3%

Protein Function

May anchor basement membranes to the underlying connective tissue.

Background

Prolargin?is a?protein?that in humans is encoded by the?PRELP?gene. It is mapped to 1q32.1. The protein encoded by this gene is a leucine-rich repeat protein present in connective tissue extracellular matrix. This protein functions as a molecule anchoring basement membranes to the underlying connective tissue. This protein has been shown to bind type I collagen to basement membranes and type II collagen to cartilage. It also binds the basement membrane heparan sulfate proteoglycan perlecan. This protein is suggested to be involved in the pathogenesis of Hutchinson-Gilford progeria (HGP), which is reported to lack the binding of collagen in basement membranes and cartilage. Alteatively spliced transcript variants encoding the same protein have been observed.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	122	112	116	125	118	5.06	4.2%
Sample 2	725	748	679	765	729	32.29	4.4%
Sample 3	2351	2384	2122	2446	2325	122.47	5.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	5549
Gene Name	PRELP
UniProt ID	P51888
Tissue Specificity	Connective tissue.

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001658	EK2110	96 wells/kit	$750		Add to Cart

Human PRELP/Prolargin ELISA Kit PicoKine®

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