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Home / ELISA Kit

Human Prefoldin subunit 5 (PFDN5) ELISA Kit

SKU : BHE13703460

Prefoldin subunit 5 is a member of the prefoldin alpha subunit family. The encoded protein is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. The complex, consisting of two alpha and four beta subunits, forms a double beta barrel assembly with six protruding coiled-coils. The encoded protein may also repress the transcriptional activity of the proto-oncogene c-Myc. Alternatively spliced transcript variants encoding different isoforms have been described.Fluorescent microscopy showed MM1 expression primarily in the nucleus, with lower intensity in nucleoli and cytoplasm. Binding analyses indicated that MM1 and c-myc bind directly and that all but the N-terminal 13 amino acids of MM1 are required for binding.

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Mfr.No.	KTE61244
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Prefoldin subunit 5 (PFDN5) ELISA Kit employs a two-site sandwich ELISA to quantitate PFDN5.
Alternative Names	PFDN5; MGC5329; MGC71907; MM-1; MM1; PFD5; c-myc binding protein; myc modulator-1; prefoldin 5
Detection Method	Colorimetric
Kit Components	Human Prefoldin subunit 5 microplate Human Prefoldin subunit 5 standard Human Prefoldin subunit 5 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Prefoldin subunit 5 (PFDN5) ELISA Kit has high sensitivity and excellent specificity for detection of Human PFDN5. No significant cross-reactivity or interference between Human PFDN5 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Prefoldin subunit 5 is a member of the prefoldin alpha subunit family. The encoded protein is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. The complex, consisting of two alpha and four beta subunits, forms a double beta barrel assembly with six protruding coiled-coils. The encoded protein may also repress the transcriptional activity of the proto-oncogene c-Myc. Alternatively spliced transcript variants encoding different isoforms have been described.Fluorescent microscopy showed MM1 expression primarily in the nucleus, with lower intensity in nucleoli and cytoplasm. Binding analyses indicated that MM1 and c-myc bind directly and that all but the N-terminal 13 amino acids of MM1 are required for binding.
Notes	This Human Prefoldin subunit 5 (PFDN5) ELISA Kit employs a two-site sandwich ELISA to quantitate PFDN5 in samples. An antibody specific for PFDN5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPFDN5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PFDN5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PFDN5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	5204

Applications	ELISA
Applications Notes	This Human Prefoldin subunit 5 (PFDN5) ELISA Kit employs a two-site sandwich ELISA to quantitate PFDN5 in samples. An antibody specific for PFDN5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPFDN5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PFDN5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PFDN5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
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