Human PRDX6 (Peroxiredoxin-6) ELISA Kit

Mfr.No.

EH1911

Sensitivity

9.375pg/ml

Detection Range

15.625-1000pg/ml

Reactivity

Human

Assay Duration

4 hours

Assay Type

Sandwich

Description

This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti PRDX6 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with PRDX6 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of PRDX6 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

Alternative Names

Peroxiredoxin-6 ELISA Kit, 1-Cys peroxiredoxin ELISA Kit, 1-Cys PRX ELISA Kit, 24 kDa protein ELISA Kit, Acidic calcium-independent phospholipase A2 ELISA Kit, aiPLA2 ELISA Kit, Antioxidant protein 2 ELISA Kit, Glutathione-dependent peroxiredoxin ELISA Kit, Liver 2D page spot 40 ELISA Kit, Lysophosphatidylcholine acyltransferase 5 ELISA Kit, LPC acyltransferase 5, LPCAT-5, Lyso-PC acyltransferase 5 ELISA Kit, Non-selenium glutathione peroxidase ELISA Kit, NSGPx ELISA Kit, Red blood cells page spot 12 ELISA Kit, PRDX6 ELISA Kit, AOP2 ELISA Kit, KIAA0106 ELISA Kit

Detection Method

Double Antibody

Kit Components

Kit Components	Item	Size(48T)	Size(96T)	Storage Condition for Opened Kit
E001	ELISA Microplate(Dismountable)	8x6	8x12	Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C
E002	Lyophilized Standard	1vial	2vial	Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C
E003	Biotin-labeled Antibody(Concentrated, 100X)	60ul	120ul	2-8°C (Avoid Direct Light)
E034	HRP-Streptavidin Conjugate(SABC, 100X)	60ul	120ul
E024	TMB Substrate	5ml	10ml
E039	Sample Dilution Buffer	10ml	20ml	2-8°C
E040	Antibody Dilution Buffer	5ml	10ml
E049	SABC Dilution Buffer	5ml	10ml
E026	Stop Solution	5ml	10ml
E038	Wash Buffer(Concentrated, 25X)	15ml	30ml
E006	Plate Sealer	3 pieces	5 pieces
E007	Product Description	1 copy	1 copy

Recovery

Add a certain amount of PRDX6 into the sample. Calculate the recovery by comparing the measured value with the expected amount of PRDX6 in the sample.

Sample Type	Recovery Range(%)	Average(%)
serum(n=10)	88-97	93
EDTA plasma(n=10)	86-104	97
Heparin plasma(n=10)	91-99	96

Linearity

Dilute the sample with a certain amount of PRDX6 at 1:2, 1:4 and 1:8 to get the recovery range.

Sample Type	1:2	1:4	1:8
serum(n=10)	88-102%	86-102%	87-94%
EDTA plasma(n=10)	88-99%	85-97%	83-101%
Heparin plasma(n=10)	81-94%	81-89%	87-98%

Specificity

Specifically binds with PRDX6 , no obvious cross reaction with other analogues.

Standard Curve

This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.) Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.

STD.(pg/ml)	OD-1	OD-2	Average
0	0.089	0.091	0.09
15.625	0.154	0.158	0.156
31.25	0.241	0.249	0.245
62.5	0.458	0.472	0.465
125	0.717	0.737	0.727
250	1.202	1.236	1.219
500	1.778	1.83	1.804
1000	2.414	2.484	2.449

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Precision

Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.

Item	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/ml)	32.9	130.6	515	31.46	126.8	508
Standard deviation	1.85	7.35	26.16	1.63	6.35	28.14
CV(%)	5.61	5.63	5.08	5.19	5.01	5.54

Background

PRDX6/aiPLA2/AOP2/NSGPx/1-Cys/24 kDa protein/Acidic calcium-independent phospholipase A2/aiPLA21-Cys PRX/Antioxidant protein 2/Non-selenium glutathione peroxidase/Red blood cells page spot 12/Liver 2D page spot 40/NSGPx1-Cys peroxiredoxin/p29/peroxiredoxin 6/PRX

Stability

Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.

ELISA kit(n=5)	37°C for 1 month	2-8°C for 6 months
Average(%)	80	95-100

Notes

For Research Use Only

Usage Notes

Step 1:
Take out the required plate wells, add 50ul Cap/Det Ab into each well, then add 50ul Standard or Sample into individual well. (When adding standard or sample, the disposable tip lightly touches the liquid level. Change the disposable tips for different samples and standards.) Gently tap the plate for 10s to ensure thorough mixing then static incubate for 60 minutes at 37°C.

Washing:
Wash the plate twice without immersion.

Step 2:
Add 100ul HRP-Streptavidin (orange) into each well, seal the plate and static incubate for 30 minutes at 37°C.

Washing:
Wash the plate five times without immersion.

Step 3:
Add 90ul TMB substrate solution, seal the plate and static incubate for 10-20 minutes at 37°C. (Accurate TMB visualization control is required.)

Step 4:
Add 50ul stop solution. Read at 450nm immediately and calculate.

UniProt ID	P30041

Applications	ELISA
Applications Notes	PRDX6 ELISA Kit allows for the in vitro quantitative determination of PRDX6 concentrations in serum, plasma, tissue homogenates and other biological fluids.

Storage	2-8°C(Sealed), Don't cryopreserve.

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SKU	Supplier No.	Size	Your price	Quantity
BHE10802034	EH1911	48T	$280		Add to Cart
BHE10802034	EH1911	96T	$400		Add to Cart

Human PRDX6 (Peroxiredoxin-6) ELISA Kit

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