Human PRAME(Melanoma antigen preferentially expressed in tumors) ELISA Kit

Mfr.No.

EH1290

Alternative Names

PRAME ELISA Kit, Melanoma antigen preferentially expressed in tumors ELISA Kit, MAPE ELISA Kit, OIP4 ELISA Kit, OIP-4 ELISA Kit, CT130 ELISA Kit, Cancer ELISA Kit, testis antigen 130 ELISA Kit, Opa-interacting protein 4 ELISA Kit, Preferentially expressed antigen of melanoma ELISA Kit

Reactivity

Human

Detection Method

Double Antibody

Detection Range

0.156-10ng/ml

Sample Type

Cell lysate, Tissue lysate, Other liquid samples, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of PRAME and the recovery rates were calculated by comparing the measured value to the expected amount of PRAME in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	87-102	94
EDTA plasma(n=5)	87-101	95
heparin plasma(n=5)	90-103	95

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PRAME and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	87-101%	89-104%	87-105%
EDTA plasma(n=5)	83-98%	82-96%	83-97%
heparin plasma(n=5)	85-99%	87-97%	82-95%

Sensitivity

< 0.094ng/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

PRAME(Melanoma antigen preferentially expressed in tumors)/MAPE/OIP4/OIP-4/CT130/Cancer,testis antigen 130/Opa-interacting protein 4/Preferentially expressed antigen of melanoma

Notes

For Research Use Only

UniProt ID	P78395
Antigen	PRAME

Applications	ELISA
Applications Notes	PRAME ELISA Kit allows for the in vitro quantitative determination of PRAME concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10802490	EH1290	96T	$400		Add to Cart

Human PRAME(Melanoma antigen preferentially expressed in tumors) ELISA Kit

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