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Home / ELISA Kit

Human PRAME family member 1 (PRAMEF1) ELISA Kit

SKU : BHE13703405

PRAMEF1, Belongs to the PRAME family.Contains 2 LRR (leucine-rich) repeats.A leucine-rich repeat (LRR) is a protein structural motif that forms an alpha/beta horseshoe fold. It is composed of repeating 2030 amino acid stretches that are unusually rich in the hydrophobic amino acid leucine. Typically, each repeat unit has beta strand-turn-alpha helix structure, and the assembled domain, composed of many such repeats, has a horseshoe shape with an interior parallel beta sheet and an exterior array of helices. One face of the beta sheet and one side of the helix array are exposed to solvent and are therefore dominated by hydrophilic residues. The region between the helices and sheets is the protein's hydrophobic core and is tightly sterically packed with leucine residues. Leucine-rich repeats are frequently involved in the formation of proteinprotein interactions.

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Mfr.No.	KTE61189
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human PRAME family member 1 (PRAMEF1) ELISA Kit employs a two-site sandwich ELISA to quantitate PRAMEF1.
Alternative Names	PRAMEF1; RP5-1198H6.2; RP5-845O24.1; dJ1198H6.1;
Detection Method	Colorimetric
Kit Components	Human PRAME family member 1 microplate Human PRAME family member 1 standard Human PRAME family member 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human PRAME family member 1 (PRAMEF1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRAMEF1. No significant cross-reactivity or interference between Human PRAMEF1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	PRAMEF1, Belongs to the PRAME family.Contains 2 LRR (leucine-rich) repeats.A leucine-rich repeat (LRR) is a protein structural motif that forms an alpha/beta horseshoe fold. It is composed of repeating 2030 amino acid stretches that are unusually rich in the hydrophobic amino acid leucine. Typically, each repeat unit has beta strand-turn-alpha helix structure, and the assembled domain, composed of many such repeats, has a horseshoe shape with an interior parallel beta sheet and an exterior array of helices. One face of the beta sheet and one side of the helix array are exposed to solvent and are therefore dominated by hydrophilic residues. The region between the helices and sheets is the protein's hydrophobic core and is tightly sterically packed with leucine residues. Leucine-rich repeats are frequently involved in the formation of proteinprotein interactions.
Notes	This Human PRAME family member 1 (PRAMEF1) ELISA Kit employs a two-site sandwich ELISA to quantitate PRAMEF1 in samples. An antibody specific for PRAMEF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRAMEF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRAMEF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRAMEF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	65121

Applications	ELISA
Applications Notes	This Human PRAME family member 1 (PRAMEF1) ELISA Kit employs a two-site sandwich ELISA to quantitate PRAMEF1 in samples. An antibody specific for PRAMEF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRAMEF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRAMEF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRAMEF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703405	KTE61189	48 T	$339		Add to Cart
BHE13703405	KTE61189	96 T	$589		Add to Cart
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BHE13703405	KTE61189	96 Tx50	$24569		Add to Cart

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