Matrices listed below were spiked with certain level of PPP1R13B and the recovery rates were calculated by comparing the measured value to the expected amount of PPP1R13B in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-103
95
EDTA plasma(n=5)
82-99
91
heparin plasma(n=5)
81-102
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PPP1R13B and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-103%
91-104%
93-103%
EDTA plasma(n=5)
92-99%
91-98%
91-96%
heparin plasma(n=5)
92-98%
91-99%
90-100%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
UniProt ID
Q96KQ4
Applications
ELISA
Applications Notes
PPP1R13B ELISA Kit allows for the in vitro quantitative determination of PPP1R13B concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human PPP1R13B (Apoptosis-stimulating of p53 protein 1) ELISA Kit