Human PPM1A(Protein Phosphatase, Mg2+/Mn2+ Dependent 1A) ELISA Kit

Mfr.No.

ELK4276

Alternative Names

PP2C-ALPHA; PP2CA; Phosphatase 2C Alpha; Protein Phosphatase 1A(Formerly 2C)Magnesium-Dependent,Alpha

Reactivity

Human

Detection Range

0.32-20 ng/mL

Sample Type

Cell lysates, Tissue homogenates, Other biological fluids

Assay Type

Sandwich

Assay Duration

3.5h

Recovery

Matrices listed below were spiked with certain level of recombinant PPM1A and the recovery rates were calculated by comparing the measured value to the expected amount of PPM1A in samples.

Matrix	Recovery range	Average
serum(n=5)	87-95%	91%
EDTA plasma(n=5)	93-107%	100%
Heparin plasma(n=5)	87-99%	93%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PPM1A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	87-96%	85-97%	85-94%	87-96%
EDTA plasma(n=5)	92-101%	82-98%	89-97%	85-93%
Heparin plasma(n=5)	86-96%	93-104%	89-97%	85-94%

Typical Data

Concentration (ng/mL)	OD	Corrected OD
20.00	2.236	2.149
10.00	1.592	1.505
5.00	1.121	1.034
2.50	0.883	0.796
1.25	0.529	0.442
0.63	0.365	0.278
0.32	0.265	0.178
0.00	0.087	0.000

Sensitivity

0.123 ng/mL

Limit Of Detection

20 ng/mL

UniProt ID	P35813
Antigen	PPM1A
Usage Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PPM1A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PPM1A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PPM1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PPM1A in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications	ELISA
Research Area	Enzyme & Kinase;
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE15202217	ELK4276	48T	$320		Add to Cart
BHE15202217	ELK4276	96T	$458		Add to Cart

Human PPM1A(Protein Phosphatase, Mg2+/Mn2+ Dependent 1A) ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Human PPM1A(Protein Phosphatase, Mg2+/Mn2+ Dependent 1A) ELISA Kit

Rate it now..!!