Matrices listed below were spiked with certain level of PPIF and the recovery rates were calculated by comparing the measured value to the expected amount of PPIF in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
91-105
98
EDTA plasma(n=5)
86-100
92
heparin plasma(n=5)
92-103
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PPIF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
91-104%
92-105%
86-98%
EDTA plasma(n=5)
87-96%
89-101%
84-100%
heparin plasma(n=5)
87-99%
84-94%
87-92%
Sensitivity
< 0.188ng/ml
Notes
For Research Use Only
UniProt ID
P30405
Antigen
PPIF
Applications
ELISA
Applications Notes
PPIF ELISA Kit allows for the in vitro quantitative determination of PPIF concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human PPIF(Peptidyl-prolyl cis-trans isomerase F) ELISA Kit