Matrices listed below were spiked with certain level of PPA1 and the recovery rates were calculated by comparing the measured value to the expected amount of PPA1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-104
95
EDTA plasma(n=5)
90-98
94
heparin plasma(n=5)
86-102
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PPA1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
PPA1 ELISA Kit allows for the in vitro quantitative determination of PPA1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human PPA1(Inorganic pyrophosphatase) ELISA Kit