Human POTE ankyrin domain family member E(POTEE) ELISA kit

Human POTE ankyrin domain family member E(POTEE) ELISA kit

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Mfr.No.

CSB-EL740863HU

Sensitivity

6.25 pg/mL

Detection Range

25 pg/mL-1600 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

POTE ankyrin domain family member E, POTE ankyrin domain family member E_x000D_ ANKRD26-like family C member 1A_x000D_ Prostate, ovary, testis-expressed protein on chromosome 2_x000D_ Short name= POTE-2_x000D_ A26C1A, POTE2, POTE ankyrin domain family member E, POTE ankyrin domain family member E_x000D_ ANKRD26-like family C member 1A_x000D_ Prostate, ovary, testis-expressed protein on chromosome 2_x000D_ Short name= POTE-2_x000D_ A26C1A, POTE2

Recovery

The recovery of mouse GDF11 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-102
EDTA plasma (n=4)	102	98-107

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse GDF11 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	85-97
1:2	Average %	89
	Range %	84-95
1:4	Average %	86
	Range %	82-93
1:8	Average %	100
	Range %	95-107

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1200	2.531	2.598	2.565	2.418
600	1.937	1.967	1.952	1.805
300	1.280	1.291	1.286	1.139
150	0.710	0.763	0.737	0.590
75	0.430	0.465	0.448	0.301
37.5	0.294	0.284	0.289	0.142
18.75	0.225	0.231	0.228	0.081
0	0.146	0.148	0.147	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q6S8J3

Applications	ELISA
Research Area	POTEE

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503842	CSB-EL740863HU	96T	$695	Add to Cart
BHE10503842	CSB-EL740863HU	5x96T	$3336	Add to Cart
BHE10503842	CSB-EL740863HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.