Human Potassium Voltage-gated Channel Subfamily E Member 1

Mfr.No.

E4851Hu

Sensitivity

10.55ng/L

Detection Range

20-3500ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Potassium Voltage-gated Channel Subfamily E Member 1 (also known as KCND1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Delayed rectifier potassium Channel subunit IsK;IKs producing slow voltage-gated potassium Channel subunit beta Mink;KCNE 1;KCNE1;Minimal potassium Channel;Potassium voltage-gated Channel subfamily E member 1

Molecular Weight

14675

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E4851Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	532	23.81	4.5
Intra-Assay	2	18	1050.8	42.35	4
Intra-Assay	3	18	1298.4	34.84	2.7

Background

Ancillary protein that assembles as a beta subunit with a voltage-gated potassium Channel complex of pore-forming alpha subunits. Modulates the gating kinetics and enhances stability of the Channel complex. Assembled with KCNB1 modulates the gating characteristics of the delayed rectifier voltage-dependent potassium Channel KCNB1 (PubMed: 19219384). Assembled with KCNQ1/KVLQT1 is proposed to form the slowly activating delayed rectifier cardiac potassium (IKs) Channel. The outward current reaches its steady state only after 50 seconds. Assembled with KCNH2/HERG may modulate the rapidly activating component of the delayed rectifying potassium current in heart (IKr).

Protocol

These standard curves of E4851Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2000ng/L	2.164	2.079	2.037
	1.994
1000ng/L	1.052	1.031	0.99
	1.011
500ng/L	0.449	0.476	0.434
	0.503
250ng/L	0.212	0.187	0.146
	0.163
125ng/L	0.1	0.11	0.069
	0.12
0ng/L	0.05	0.041	0
	0.033

Gene ID	3753
UniProt ID	P15382

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106126	E4851Hu	48T	$320		Add to Cart
BHE12106126	E4851Hu	96T	$458		Add to Cart

Human Potassium Voltage-gated Channel Subfamily E Member 1 - KCNE1 ELISA Kit

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