Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of PORCN and the recovery rates were calculated by comparing the measured value to the expected amount of PORCN in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-100
93
EDTA plasma(n=5)
85-101
92
heparin plasma(n=5)
88-94
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PORCN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
PORCN ELISA Kit allows for the in vitro quantitative determination of PORCN concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human PORCN (Probable protein-cysteine N-palmitoyltransferase porcupine) ELISA Kit