Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of POP7 and the recovery rates were calculated by comparing the measured value to the expected amount of POP7 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-100
93
EDTA plasma(n=5)
88-98
94
heparin plasma(n=5)
94-102
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of POP7 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-102%
85-105%
92-103%
EDTA plasma(n=5)
86-97%
90-101%
84-97%
heparin plasma(n=5)
87-99%
81-97%
83-89%
Sensitivity
< 46.875pg/ml
Notes
For Research Use Only
UniProt ID
O75817
Antigen
POP7
Applications
ELISA
Applications Notes
POP7 ELISA Kit allows for the in vitro quantitative determination of POP7 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human POP7 (Ribonucleases P/MRP protein subunit POP7 homolog) ELISA Kit