Matrices listed below were spiked with certain level of POMC and the recovery rates were calculated by comparing the measured value to the expected amount of POMC in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-99
95
EDTA plasma(n=5)
85-104
93
heparin plasma(n=5)
91-100
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of POMC and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
POMC ELISA Kit allows for the in vitro quantitative determination of POMC concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human POMC(Pro-opiomelanocortin) ELISA Kit