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Human Polypeptide N-acetylgalactosaminyltransferase 12,GALNT12 ELISA Kit

BHE12107261

Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has activity toward non-glycosylated peptides such as Muc5AC, Muc1a and EA2, and no detectable activity with Muc2 and Muc7. Displays enzymatic activity toward the Gal-NAc-Muc5AC glycopeptide, but no detectable activity to mono-GalNAc-glycosylated Muc1a, Muc2, Muc7 and EA2. May play an important role in the initial step of mucin-type oligosaccharide biosynthesis in digestive organs.

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+1 866.986.9598
Email
[email protected]
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Gene ID 79695
UniProt ID Q8IXK2
Antigen GALNT12
Research Area Tags & Cell Markers
Protocol These standard curves of E5899Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.031 2.064 2.021
2.098
600ng/L 1.843 1.796 1.752
1.749
300ng/L 1.247 1.23 1.186
1.213
150ng/L 0.665 0.686 0.642
0.707
75ng/L 0.35 0.365 0.321
0.38
0ng/L 0.051 0.044 0
0.036
Precision We measured random samples of E5899Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 180.8 8.22 4.55
Intra-Assay 2 18 554.16 39.72 7.17
Intra-Assay 3 18 131.17 6.74 5.14
Storage 2-8°C
SKU Supplier No. Size Your price Quantity
BHE12107261 E5899Hu 48T $320
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BHE12107261 E5899Hu 96T $458
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