Human Polycomb complex protein BMI-1(BMI1) ELISA kit

Human Polycomb complex protein BMI-1(BMI1) ELISA kit

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Mfr.No.

CSB-EL002726HU

Sensitivity

6.25 pg/mL

Detection Range

25 pg/mL-1600 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

BMI1 polycomb ring finger oncogene, RP11-573G6.1, MGC12685, PCGF4, RNF51, B lymphoma Mo-MLV insertion region 1 homolog, flvi-2/bmi-1, murine leukemia viral (bmi-1) oncogene homolog, oncogene BMI-1, polycomb group protein Bmi1, polycomb gro

Recovery

The recovery of mouse PDGFsR-? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	88-98
EDTA plasma (n=4)	99	96-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PDGFsR-? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	86-93
1:2	Average %	97
	Range %	92-101
1:4	Average %	91
	Range %	88-97
1:8	Average %	92
	Range %	84-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.795	3.012	2.904	2.787
500	2.214	2.304	2.259	2.142
250	1.436	1.550	1.493	1.376
125	0.899	0.974	0.937	0.820
62.5	0.472	0.461	0.467	0.350
31.25	0.351	0.342	0.347	0.230
15.6	0.244	0.253	0.249	0.132
0	0.113	0.120	0.117

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P35226

Applications	ELISA
Research Area	BMI1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503835	CSB-EL002726HU	96T	$695	Add to Cart
BHE10503835	CSB-EL002726HU	5x96T	$3336	Add to Cart
BHE10503835	CSB-EL002726HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.