Human Polyamine-Modulated Factor 1-binding Protein 1

Mfr.No.

E3187Hu

Sensitivity

9.58ng/L

Detection Range

20-4500ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

PMF-1-binding protein;PMFBP 1;PMFBP1;Polyamine-modulated factor 1-binding protein 1

Molecular Weight

117494

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E3187Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	177.1	11.75	6.6
Intra-Assay	2	18	573.3	43.72	7.6
Intra-Assay	3	18	813.6	41.4	5.1

Background

Required for normal spermatogenesis (PubMed: 1770140, PubMed: 30032984, PubMed: 30298696). It functions as a scaffold protein that attaches the sperm head-tail connecting piece to the nuclear envelope, thus maintaining sperm head and tail integrity (PubMed: 30032984). May also be involved in the general organization of cellular cytoskeleton (By similarity).

Protocol

These standard curves of E3187Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.073	2.103	2.054
	2.133
1200ng/L	1.613	1.645	1.596
	1.677
600ng/L	1.008	0.989	0.94
	0.971
300ng/L	0.784	0.777	0.728
	0.771
150ng/L	0.42	0.413	0.363
	0.405
0ng/L	0.052	0.049	0
	0.047

Gene ID	83449
UniProt ID	Q8TBY8

Research Area	Epigenetics and Nuclear Signaling

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104716	E3187Hu	48T	$320		Add to Cart
BHE12104716	E3187Hu	96T	$458		Add to Cart

Human Polyamine-Modulated Factor 1-binding Protein 1 - PMFBP1 ELISA Kit

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