Human Poly ADP ribose polymerase,PARP ELISA Kit

Human Poly ADP ribose polymerase,PARP ELISA Kit

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Mfr.No.

CSB-E10705h

Sensitivity

3.9 pg/mL

Detection Range

15.6 pg/mL-1000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

poly (ADP-ribose) polymerase 1, RP11-125A15.2, ADPRT, ADPRT1, PARP, PARP-1, PPOL, pADPRT-1, ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase), ADP-ribosyltransferase NAD(+), poly (ADP-ribose) polymerase family, member 1, po

Recovery

The recovery of human CNN2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	98-106
EDTA plasma (n=4)	94	86-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CNN2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	81-89
1:2	Average %	98
	Range %	93-103
1:4	Average %	95
	Range %	89-98
1:8	Average %	97
	Range %	92-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.325	2.413	2.369	2.254
1000	1.876	1.941	1.909	1.794
500	1.374	1.282	1.328	1.213
250	0.752	0.764	0.758	0.643
125	0.434	0.441	0.438	0.323
62.5	0.282	0.297	0.290	0.175
31.25	0.183	0.187	0.185	0.070
0	0.114	0.115	0.115

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P09874

Applications	ELISA
Research Area	PARP1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
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BHE10503833	CSB-E10705h	5x96T	$3792	Add to Cart
BHE10503833	CSB-E10705h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.