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Home / ELISA Kit

Human Poly (A) RNA polymerase, mitochondrial (MTPAP) ELISA Kit

SKU : BHE13703695

Mitochondrial poly(A) polymerase encoded by MTPAP is a member of the DNA polymerase type-B-like family. This enzyme synthesizes the 3' poly(A) tail of mitochondrial transcripts and plays a role in replication-dependent histone mRNA degradation.

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Abbkine Scientific Co., Ltd.

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Mfr.No.	KTE61480
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Poly (A) RNA polymerase, mitochondrial (MTPAP) ELISA Kit employs a two-site sandwich ELISA to quantitate MTPAP.
Alternative Names	MTPAP; FLJ10486; PAPD1; RP11-305E6.3; PAP associated domain containing 1
Detection Method	Colorimetric
Kit Components	Human Poly microplate Human Poly standard Human Poly detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Poly (A) RNA polymerase, mitochondrial (MTPAP) ELISA Kit has high sensitivity and excellent specificity for detection of Human MTPAP. No significant cross-reactivity or interference between Human MTPAP and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Mitochondrial poly(A) polymerase encoded by MTPAP is a member of the DNA polymerase type-B-like family. This enzyme synthesizes the 3' poly(A) tail of mitochondrial transcripts and plays a role in replication-dependent histone mRNA degradation.
Notes	This Human Poly (A) RNA polymerase, mitochondrial (MTPAP) ELISA Kit employs a two-site sandwich ELISA to quantitate MTPAP in samples. An antibody specific for MTPAP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMTPAP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTPAP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTPAP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	55149

Applications	ELISA
Applications Notes	This Human Poly (A) RNA polymerase, mitochondrial (MTPAP) ELISA Kit employs a two-site sandwich ELISA to quantitate MTPAP in samples. An antibody specific for MTPAP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMTPAP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTPAP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTPAP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703695	KTE61480	48 T	$339		Add to Cart
BHE13703695	KTE61480	96 T	$589		Add to Cart
BHE13703695	KTE61480	96 Tx5	$2629		Add to Cart
BHE13703695	KTE61480	96 Tx50	$24569		Add to Cart

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