Human Podoplanin(PDPN) ELISA kit

Human Podoplanin(PDPN) ELISA kit

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Mfr.No.

CSB-EL017739HU

Alternative Names

podoplanin, CTA-520D8.1, AGGRUS, GP36, GP40, Gp38, HT1A-1, OTS8, PA2.26, T1A, T1A-2, glycoprotein, 36-KD, hT1alpha-1, hT1alpha-2, lung type I cell membrane associated glycoprotein, lung type-I cell membrane-associa

Reactivity

Human

Detection Range

25 pg/mL-1600 pg/mL

Sample Type

Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse IL-31 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	93-104
EDTA plasma (n=4)	99	90-106

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-31 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	82-99
1:2	Average %	97
	Range %	87-105
1:4	Average %	95
	Range %	88-100
1:8	Average %	90
	Range %	82-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.886	2.846	2.866	2.702
1000	2.347	2.336	2.342	2.178
500	1.559	1.602	1.581	1.417
250	0.897	0.878	0.888	0.724
125	0.532	0.557	0.545	0.381
62.5	0.366	0.374	0.370	0.206
31.25	0.262	0.245	0.254	0.090
0	0.162	0.165	0.164	?

Species

Homo sapiens

Sensitivity

6.25 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q86YL7
Antigen	Podoplanin

Applications

ELISA

Research Area

PDPN

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503831	CSB-EL017739HU	96T	$695	Add to Cart
BHE10503831	CSB-EL017739HU	5x96T	$3336	Add to Cart
BHE10503831	CSB-EL017739HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.