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Home / ELISA Kit

Human Podocin - PDCN ELISA Kit

SKU : BHE12107255

Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton.

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Mfr.No.

E5893Hu

Sensitivity

0.056ng/mL

Detection Range

0.1-40ng/mL

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

NPHS 2;NPHS2;Podocin

Molecular Weight

42201

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5893Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	16.9	0.81	4.8
Intra-Assay	2	18	2.3	0.13	5.8
Intra-Assay	3	18	6.8	0.38	5.6

Background

Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton.

Protocol

These standard curves of E5893Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/mL	2.238	2.17	2.118
	2.102
12ng/mL	1.555	1.509	1.457
	1.463
6ng/mL	0.926	0.93	0.878
	0.933
3ng/mL	0.521	0.507	0.456
	0.493
1.5ng/mL	0.273	0.281	0.23
	0.289
0ng/mL	0.043	0.051	0
	0.06

Gene ID	7827
UniProt ID	Q9NP85

Research Area	Signal Transduction

Storage	2-8°C

Reference	Urinary and Kidney Podocalyxin and Podocin Levels in Diabetic Kidney Disease: A Kidney Biopsy Study Journal: Kidney Med. 2022 Nov 14;5(1):100569.

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107255	E5893Hu	48T	$320		Add to Cart
BHE12107255	E5893Hu	96T	$458		Add to Cart

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