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Home / ELISA Kit

Human Podocalyxin (PCX) ELISA Kit

SKU : BHE13703498

Podocalyxin belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology. Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors. It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells.The protein was originally identified as an important component of glomerular podocytes. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin.

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Mfr.No.	KTE61282
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Podocalyxin (PCX) ELISA Kit employs a two-site sandwich ELISA to quantitate PCX.
Alternative Names	PCX
Detection Method	Colorimetric
Kit Components	Human Podocalyxin microplate Human Podocalyxin standard Human Podocalyxin detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Podocalyxin (PCX) ELISA Kit has high sensitivity and excellent specificity for detection of Human PCX. No significant cross-reactivity or interference between Human PCX and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Podocalyxin belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology. Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors. It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells.The protein was originally identified as an important component of glomerular podocytes. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin.
Notes	This Human Podocalyxin (PCX) ELISA Kit employs a two-site sandwich ELISA to quantitate PCX in samples. An antibody specific for PCX has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPCX present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCX is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCX bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Podocalyxin (PCX) ELISA Kit employs a two-site sandwich ELISA to quantitate PCX in samples. An antibody specific for PCX has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPCX present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCX is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCX bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703498	KTE61282	48 T	$359		Add to Cart
BHE13703498	KTE61282	96 T	$619		Add to Cart
BHE13703498	KTE61282	96 Tx5	$2809		Add to Cart
BHE13703498	KTE61282	96 Tx50	$26209		Add to Cart

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