Matrices listed below were spiked with certain level of PNOC and the recovery rates were calculated by comparing the measured value to the expected amount of PNOC in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-105
100
EDTA plasma(n=5)
85-104
97
heparin plasma(n=5)
88-100
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PNOC and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
PNOC ELISA Kit allows for the in vitro quantitative determination of PNOC concentrations in serum, plasma, tissue homogenates and other biological fluids.