Matrices listed below were spiked with certain level of PLXNB1 and the recovery rates were calculated by comparing the measured value to the expected amount of PLXNB1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-100
96
EDTA plasma(n=5)
87-99
95
heparin plasma(n=5)
86-101
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PLXNB1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
89-103%
90-104%
85-105%
EDTA plasma(n=5)
87-99%
82-101%
82-101%
heparin plasma(n=5)
85-99%
84-96%
82-100%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
O43157
Applications
ELISA
Applications Notes
PLXNB1 ELISA Kit allows for the in vitro quantitative determination of PLXNB1 concentrations in serum, plasma, tissue homogenates and other biological fluids.