Human Plexin B3 ELISA Kit PicoKine®

Mfr.No.

EK1827

Sensitivity

<15 pg/ml

Detection Range

93.6 pg/ml - 6,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	93.8	187.5	375	750	1500	3000	6000
O.D.	0.021	0.110	0.177	0.334	0.587	0.994	1.635	2.389

Description

Human Plexin B3 ELISA Kit PicoKine® (96 Tests). Quantitate Human PLXNB3 in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Plexin-B3; PLXNB3; KIAA1206; PLXN6

Immunogen

Expression system for standard: NS0; Immunogen sequence: H45-Q1255

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Plexin B3

Subcellular Localization

Cell membrane, Single-pass type I membrane protein

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	156	984	3201	169	992	3321
Standard deviation	9.52	53.14	208.07	7.94	69.44	259.04
CV (%)	6.1%	5.4%	6.5%	4.7%	7.0%	7.8%

Protein Function

Receptor for SEMA5A that plays a role in axon guidance, invasive growth and cell migration. Stimulates neurite outgrowth and mediates Ca2+/Mg2+-dependent cell aggregation. In glioma cells, SEMA5A stimulation of PLXNB3 results in the disassembly of F-actin

Background

Plexin-B3 is a protein that in humans is encoded by the PLXNB3 gene. Ths gene is mapped to Xq28. The protein encoded by this gene is a member of the plexin family. It functions as a receptor for semaphorin 5A, and plays a role in axon guidance, invasive growth and cell migration. Alteatively spliced transcript variants encoding different isoforms have been found for this gene.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	156	141	147	153	149	5.76	3.8%
Sample 2	984	848	932	931	923	48.70	5.2%
Sample 3	3201	3180	3318	2761	3115	211.03	6.7%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	5365
Gene Name	PLXNB3
UniProt ID	Q9ULL4
Tissue Specificity	Expression detected in Purkinje and granular cells in cerebellum, and in brain neocortex but not in corpus callosum. Expressed in glioma cells and embryonic kidney cells (at protein level). Expressed in brain, liver, pancreas and placenta, with weak expre

Applications	ELISA
Research Area	Axonal Guidance Proteins,Growth and Development,Neurology Process,Neuroscience

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001415	EK1827	96 wells/kit	$750		Add to Cart

Human Plexin B3 ELISA Kit PicoKine®

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