Human pleiotrophin,PTN ELISA Kit

Human pleiotrophin,PTN ELISA Kit

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Mfr.No.

CSB-E09305h

Sensitivity

0.06 ng/mL

Detection Range

0.24 ng/mL-15 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

pleiotrophin, HARP, HBGF8, HBNF, NEGF1, heparin affin regulatory protein, heparin binding growth factor 8, heparin-binding growth-associated molecule, neurite growth-promoting factor 1, pleiotrophin (heparin binding

Recovery

The recovery of mouse ANG-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	97	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ANG-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:20	Average %	92
	Range %	88-95
1:40	Average %	105
	Range %	100-107
1:80	Average %	93
	Range %	87-97
1:160	Average %	97
	Range %	91-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
10	2.427	2.411	2.419	2.250
5	1.988	2.022	2.005	1.836
2.5	1.354	1.379	1.367	1.198
1.25	0.780	0.742	0.761	0.592
0.625	0.495	0.464	0.480	0.311
0.312	0.362	0.377	0.370	0.201
0.156	0.265	0.255	0.260	0.091
0	0.171	0.167	0.169	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P21246

Applications	ELISA
Research Area	PTN

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503824	CSB-E09305h	96T	$835	Add to Cart
BHE10503824	CSB-E09305h	5x96T	$4008	Add to Cart
BHE10503824	CSB-E09305h	10x96T	$7932.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.