Matrices listed below were spiked with certain level of PLB and the recovery rates were calculated by comparing the measured value to the expected amount of PLB in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-100
97
EDTA plasma(n=5)
87-104
93
heparin plasma(n=5)
85-104
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PLB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
PLB ELISA Kit allows for the in vitro quantitative determination of PLB concentrations in serum, plasma, tissue homogenates and other biological fluids.