Human Platelet Factor 4 - PF-4 ELISA Kit

Mfr.No.

E1108Hu

Description

This sandwich kit is for the accurate quantitative detection of Human Platelet Factor 4 (also known as Platelet Factor 4) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

C-X-C motif chemokine 4;Endothelial cell growth inhibitor;Iroplact;Oncostatin-A;PF 4;PF4;PF-4;Platelet factor 4;Platelet factor 4, short form;

Reactivity

Human

Detection Range

0.05-20ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

10845

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.025ng/ml

Background

Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form.

Gene ID	5196
UniProt ID	P02776
Antigen	PF4

Research Area

Cardiovascular

Protocol

These standard curves of E1108Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	1.726	1.77	1.713
	1.814
6ng/ml	0.929	1.016	0.96
	1.104
3ng/ml	0.568	0.568	0.512
	0.569
1.5ng/ml	0.164	0.223	0.166
	0.282
0.75ng/ml	0.125	0.11	0.053
	0.094
0ng/ml	0.052	0.057	0
	0.061

Precision

We measured random samples of E1108Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	6	0.2	3.4
Intra-Assay	2	18	7.9	0.24	3.1
Intra-Assay	3	18	4.1	0.21	5

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102756	E1108Hu	48T	$320		Add to Cart
BHE12102756	E1108Hu	96T	$458		Add to Cart

Human Platelet Factor 4 - PF-4 ELISA Kit

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