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Home / ELISA Kit

Human Platelet-derived growth factor D (PDGFD) ELISA Kit

SKU : BHE13703485

Platelet-derived growth factor D is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines, seven of which are found in this factor.
This gene product only forms homodimers and, therefore, does not dimerize with the other three family members. It differs from alpha and beta members of this family in having an unusual N-terminal domain, the CUB domain. Two splice variants have been identified for this gene.

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Mfr.No.	KTE61269
Reactivity	Human
Sample Type	Serum, Cell culture supernatants, Plasma, Other biological fluids
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Platelet-derived growth factor D (PDGFD) ELISA Kit employs a two-site sandwich ELISA to quantitate PDGFD.
Alternative Names	PDGFD; IEGF; MGC26867; MSTP036; SCDGF-B; SCDGFB; iris-expressed growth factor; spinal cord derived growth factor B; spinal cord-derived growth factor-B
Detection Method	Colorimetric
Kit Components	Human Platelet-derived growth factor D microplate Human Platelet-derived growth factor D standard Human Platelet-derived growth factor D detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Platelet-derived growth factor D (PDGFD) ELISA Kit has high sensitivity and excellent specificity for detection of Human PDGFD. No significant cross-reactivity or interference between Human PDGFD and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Platelet-derived growth factor D is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines, seven of which are found in this factor. This gene product only forms homodimers and, therefore, does not dimerize with the other three family members. It differs from alpha and beta members of this family in having an unusual N-terminal domain, the CUB domain. Two splice variants have been identified for this gene.
Notes	This Human Platelet-derived growth factor D (PDGFD) ELISA Kit employs a two-site sandwich ELISA to quantitate PDGFD in samples. An antibody specific for PDGFD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDGFD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDGFD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDGFD bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	80310
Antigen	PDGFD

Applications	ELISA
Applications Notes	This Human Platelet-derived growth factor D (PDGFD) ELISA Kit employs a two-site sandwich ELISA to quantitate PDGFD in samples. An antibody specific for PDGFD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDGFD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDGFD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDGFD bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703485	KTE61269	48 T	$339		Add to Cart
BHE13703485	KTE61269	96 T	$589		Add to Cart
BHE13703485	KTE61269	96 Tx5	$2629		Add to Cart
BHE13703485	KTE61269	96 Tx50	$24569		Add to Cart

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