Human Platelet-Derived Growth Factor-BB,PDGF-BB ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08923h

Sensitivity

7.81 pg/mL

Detection Range

31.25 pg/mL-2000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Recovery

The recovery of canine FGF23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-95
EDTA plasma (n=4)	93	90-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of canine FGF23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	95-101
1:2	Average %	87
	Range %	83-91
1:4	Average %	98
	Range %	94-102
1:8	Average %	84
	Range %	81-86

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	PDGF-BB

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Low temperature plasma induces angiogenic growth factor via up-regulating hypoxia-inducible factor 1a in human dermal fibroblasts Hui SongCui.et al,Archives of Biochemistry and Biophysics,Jul 24

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