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Home / ELISA Kit

Human Platelet-activating factor receptor (PTAFR) ELISA Kit

SKU : BHE13703293

The platelet-activating factor receptor is a G-protein coupled receptor which binds platelet-activating factor.The PAF receptor shows structural characteristics of the rhodopsin gene family and binds platelet-activating factor (PAF). PAF is a phospholipid (1-0-alkyl-2-acetyl-sn-glycero-3-phosphorylcholine) that has been implicated as a mediator in diverse pathologic processes, such as allergy, asthma, septic shock, arterial thrombosis, and inflammatory processes.
PTAFR has 2 N-glycosylation sites, but unlike other rhodopsin family members, there is no N-glycosylation site in the N-terminal segment. Transfection of PTAFR into COS-7 cells resulted in expression of the receptor with an extracellular N terminus.

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Mfr.No.	KTE61077
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Platelet-activating factor receptor (PTAFR) ELISA Kit employs a two-site sandwich ELISA to quantitate PTAFR.
Alternative Names	PTAFR; PAFR; OTTHUMP00000004041
Detection Method	Colorimetric
Kit Components	Human Platelet-activating factor receptor microplate Human Platelet-activating factor receptor standard Human Platelet-activating factor receptor detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Platelet-activating factor receptor (PTAFR) ELISA Kit has high sensitivity and excellent specificity for detection of Human PTAFR. No significant cross-reactivity or interference between Human PTAFR and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The platelet-activating factor receptor is a G-protein coupled receptor which binds platelet-activating factor.The PAF receptor shows structural characteristics of the rhodopsin gene family and binds platelet-activating factor (PAF). PAF is a phospholipid (1-0-alkyl-2-acetyl-sn-glycero-3-phosphorylcholine) that has been implicated as a mediator in diverse pathologic processes, such as allergy, asthma, septic shock, arterial thrombosis, and inflammatory processes. PTAFR has 2 N-glycosylation sites, but unlike other rhodopsin family members, there is no N-glycosylation site in the N-terminal segment. Transfection of PTAFR into COS-7 cells resulted in expression of the receptor with an extracellular N terminus.
Notes	This Human Platelet-activating factor receptor (PTAFR) ELISA Kit employs a two-site sandwich ELISA to quantitate PTAFR in samples. An antibody specific for PTAFR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTAFR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTAFR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTAFR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	5724

Applications	ELISA
Applications Notes	This Human Platelet-activating factor receptor (PTAFR) ELISA Kit employs a two-site sandwich ELISA to quantitate PTAFR in samples. An antibody specific for PTAFR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTAFR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTAFR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTAFR bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703293	KTE61077	48 T	$339		Add to Cart
BHE13703293	KTE61077	96 T	$589		Add to Cart
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BHE13703293	KTE61077	96 Tx50	$24569		Add to Cart

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